The old Glazkovskoye suburb, although younger than Irkutsk, has been known since the late 17th century. Some believe that Irkutsk actually began here, recalling the wintering site on Dyachy Island. However, until the early 19th century, when the overland Krugomorsk tract was created, the suburb remained sparsely populated and poor, belonging to the parish of the Trinity Church across the Angara River, which made access to it difficult in the off-season. In the early 19th century, the residents of Glazkovskoye suburb wished to build their own church. In 1811, Irkutsk merchant N.S. Chupalov proposed to build a stone church in the name of St. Nicholas in the village of Mogilevo, but his initiative was not realized, and Glazkovskoye remained without a church for another fifty years.

On November 14, 1857, the residents of the suburb appealed to the Irkutsk and Nerchinsk Bishop Eusebius with a request to build a stone church. The elder of the Trinity Church, Y.S. Malkov, purchased a plot in the center of the suburb, prepared materials, and took on the expenses for construction, including a house for the priest. The residents requested a blessing for the construction of a single-altar church with a wooden bell tower in the name of the saints Nicholas and Innocent, along with the organization of a cemetery. On December 8 of the same year, Eusebius agreed to the construction, noting the need to coordinate the plan with the provincial construction commission and architect.

In the spring of 1858, approvals were obtained, and on April 27, the church was laid. Before this, a liturgy and a procession took place. The importance of the event was emphasized by the participation of high-ranking officials. The church was built in a year and a half.

On September 18, 1859, it was reported that the construction was completed and the church was ready for consecration. On September 20, the church was consecrated by Eusebius. The construction cost Y.S. Malkov 10,000 rubles, and an additional 2,000 rubles for utensils and books came from benefactors. The church was built in the style of classicism with brick walls and modest decor.

Four years later, on June 16, 1863, the construction of an altar in the name of the Assumption of the Mother of God began with funds from I.S. Mogilev. The consecration took place on April 28, 1866. The altar disrupted the symmetry of the building but had a separate entrance and a semicircular apse. Subsequently, only routine repairs were carried out in the church.

In 1883, the church was decorated inside, and an arch was pierced, uniting both churches. The work cost 1,200 rubles, financed by parishioners and a subsidy. Before World War I, an expansion of the church was planned, but the war and subsequent events hindered this.

The policy of atheism and economic difficulties led to the decline of the church. In 1932, church property was looted, and in 1933, the bells were removed. In 1934, the church was closed, and the building was repurposed as a club, and later a cinema called 'Zarya.' In 1979, the cinema was closed, and the building was abandoned.

In 1990, the building was recognized as a monument, and in 1994, its restoration began. In 1997, the building was returned to the Irkutsk Patriarchate, and archaeological work commenced. In 2000, it was decided to build a new church on the same site. In 2001, construction began, completed in 2003.

The new building corresponds to the original but includes a basement and a light dome. The bell tower was adorned with new bells. Modern communications and heating were installed in the church. In 2003, the consecration of all three altars of the church took place.