Until 1871, a third-class women's monastery operated at the Vvedensko-Nikolayevskaya Church, the foundation of which is unknown, but it existed as early as the beginning of the 17th century. The monastery church of St. Nicholas was mentioned in the patriarchal books of 1628. In 1666, a new stone church was built in the monastery in honor of St. Nikon of Radonezh, with an altar dedicated to St. Nicholas, while a separate wooden Nicholas church was abolished. In 1685, a separate wooden church of St. Nicholas the Wonderworker was rebuilt, which stood until 1710 when it burned down in a fire in Yuriev. In this same fire, the wooden monastery church of the Introduction of the Blessed Virgin Mary was also destroyed, with only the Nikon church surviving. In 1725, a stone altar in honor of the Introduction of the Blessed Virgin Mary was added to it, but between 1763 and 1766, at the initiative of Abbess Agafiya and the parishioners, a new stone five-domed cathedral was built in honor of the Introduction of the Virgin Mary. After this, the Vvedensky altar in the Nikon church was renamed to Nikolayevsky.

In 1785, thanks to merchant Kartsev, the monastery was surrounded by a stone fence on three sides, and a wooden fence on the northern side. Between 1811 and 1826, a new stone four-tiered bell tower with gates underneath was built to replace the old one. In 1856, Abbess Pulcheria replaced the wooden fence with a stone enclosure.

In 1871, a fire destroyed the Vvedensky monastery, and it was not restored. The nuns were distributed to other monasteries, and the restored Vvedensky church became a parish church.

After the revolution, the churches were closed, turned into a warehouse and workshops, and later the buildings were occupied by a bread factory.

On December 24, 2000, the Holy Synod reopened the monastery as a men's monastery. Restoration work is currently underway on its territory.