The village of Bolshoye Mikushkino was founded in 1756 by Chuvash people who migrated from various districts of the Volga region. Its population consisted of state peasants.

In the 19th century, the village was part of the Buguruslan district, initially in the Orenburg province, and later in the Samara province. From the second half of the 19th century, Russians also began to settle here.

In the mid-18th century, an Orthodox church was opened in the village, which soon burned down. In 1871, a church in honor of the Holy Trinity was built in Bolshoye Mikushkino on the site of the lost temple.

In the 1920s, the Trinity Church was closed, and the further fate of the building is unknown.

On June 19, 2008, a parish in honor of the Holy Trinity was re-registered in the village. A new wooden church was built from 2009 to 2012 with funds from a native of the Isaklinsky district, Moiseev A.I. A memorial stone on the church grounds indicates that it was erected in memory of the victims of repression in the 1930s-1940s and participants of the Great Patriotic War who perished in 1941-1945. The bells for the church were made and installed with donations from parishioners.