The first reliable mentions of the Borisogleb Monastery are found in the Nikon and Novgorod Fourth Chronicles and date back to the first half of the 14th century. The Vladimir Chronicle for the year 1346 states: "That same year, Prince Vasily Yaroslavich of Murom passed away, in the church and in the skeme, he was laid to rest in Murom, in the church of the holy martyrs Boris and Gleb." The Nikon Chronicle adds: "in the monastery on Ushin, under Metropolitan Theognost." The Novgorod Fourth Chronicle specifies that Prince Vasily Yaroslavich died in the winter of the year 6853, that is, at the end of 1345.

For a long time, the monastery remained wooden, and only in the 17th century were new stone churches built on the site of the old wooden structures: in honor of the Ascension of the Lord with a chapel dedicated to the martyrs Boris and Gleb, and in honor of the Nativity of Christ and the Kazan Icon of the Most Holy Theotokos. The exact date of their construction is unknown, but from an inscription on the preserved antimins, it follows that the altar of the chapel in the name of the martyrs Boris and Gleb was consecrated in 1681. In the revenue books of the Ryazan Diocese for the year 1676, to which the Murom district then belonged, the church of the martyrs Boris and Gleb is mentioned in the Borisogleb Monastery. "By that church is the yard of priest Mikhail, deacon Zotika, and sacristan Stepan, in the parish 5 service yards, 59 peasant yards, and 19 landless; the village of Blagoveshchenskoye, with 30 peasant yards; the village of Pentsy, with 6 peasant yards, and the village of Varezh, with 32 peasant yards and 7 landless." The monastery was surrounded by a stone fence.

In 1992, the Church of the Nativity of Christ of the former Borisogleb Monastery was returned to the Russian Orthodox Church and became a dependency of the Holy Blagoveshchensky Men's Monastery in the city of Murom. In 2013, with the blessing of Bishop Nila of Murom and Vyazniki, the Church of the Nativity of Christ became an Archierarchical dependency. Monastic life resumed, and services became regular. Currently, services are held regularly in the Church of the Nativity of Christ on the first floor of the monastery. On the second floor is the church in honor of the Kazan Icon of the Mother of God. A pilgrimage center "Evdokia" has been opened at the dependency, named after the daughter of Saints Peter and Fevronia of Murom, designed for 80 people. There is also a refectory, a museum of "Russian Costume and Everyday Life", a library, a spring, and a bathing area, along with the monastery's economy.