The village of Frolovskoye, previously known as Florovskoye, is one of the oldest settlements in the Yuryyevsky district. It is mentioned in the spiritual charter of 1389 by Grand Prince Dmitry Ivanovich Donskoy, who bequeathed it to his wife, Princess Evdokia Dmitrievna. They had eight sons: Daniil, Vasily, Yuri, Semyon, Andrey, Pyotr, Ivan, and Konstantin, as well as four daughters: Sofia, Maria, Anastasia, and Anna. The eldest son, Grand Prince Vasily Dmitrievich, exchanged the village with his mother and in 1426 bequeathed it to his wife, Princess Sofia Vitovtovna, the daughter of the Grand Duke of Lithuania Vitovt, whom he married in 1391. They had five sons: Yuri, Ivan, Daniil, Semyon, and Vasily, and four daughters: Anna, Anastasia, Maria, and Vasilisa. In 1462, Grand Prince Vasily Vasilievich bequeathed the village to his wife Maria Yaroslavovna. After her death, their youngest son, Prince Boris Vasilievich Volotsky, inherited it. In 1471, Boris married Princess Ulyana Mikhailovna, the daughter of the appanage prince of Kholm, Mikhail Dmitrievich. They had sons Fyodor and Ivan. In the early 16th century, the village passed to Fyodor Borisovich, the son of Prince Boris, who died childless. After his death, the village was managed by his widow Maria. Florovskoye remained a palace village until 1691, when it was transferred to the boyars Pyotr and Vasily Abramovich Lopukhin.

The church in Florovskoye existed since the 14th century. In 1628, documents from the Patriarchal Treasury mention the 'Church of John the Theologian in the royal palace village of Frolovskoye.' In 1691, it was described as 'dilapidated.' In 1828, the wooden church was struck by lightning and burned down, and the construction of a stone church began the same year. The current stone church of the Holy Apostle and Evangelist John the Theologian, with chapels of the Kazan Icon of the Mother of God and St. Nicholas of Myra, was consecrated in 1838. The stone bell tower was built in 1852.

During the Soviet era, the temple was closed and desecrated, with its roof, altar, bell tower, and church fence destroyed.