The Blagoveshchensky Women's Monastery was founded in the early 17th century, with the first mention in 1614, and was located in the territory of the White City. After the abolition of the Ascension Men's Monastery in 1706, the convent was moved to its territory, where a stone Blagoveshchensky church, consecrated in 1699, already stood. In the early 17th century, an altar was added to the church in honor of the Martyr Paraskeva Friday. In 1722, an ancient wooden voivodeship church in honor of the Apostle John the Theologian was transferred from the Kremlin to the monastery grounds, where an altar was arranged for the icon of the Mother of God "Life-Giving Spring." Later, the church was rebuilt in stone with funds from Bishop Mephodiy and was consecrated on March 19, 1765. In the second half of the 19th century, due to its dilapidation, the church was significantly reconstructed and was re-consecrated on October 30, 1865. A separate chapel was built for the icon of the Mother of God "Life-Giving Spring," where the miraculous image was placed.

In 1778, Abbess Margarita arranged a small stone chapel in the southeastern corner of the monastery, where the revered icon of St. Nicholas the Wonderworker, honored by the Astrakhan people, was placed. By the end of the 19th century, the chapel had fallen into disrepair and was almost destroyed. In 1900, with the blessing of Abbess Zinaida, the chapel was dismantled and the construction of a new one began, which was consecrated in February 1901.

In the early 20th century, a new monastery bell tower was erected on a plot of land acquired from the city, with construction completed in September 1907.

The persecution of the monastery began in late 1918, and it was finally closed in 1922. In 1929, the building of the John the Theologian Church, along with the "Life-Giving Spring" chapel, was sold for demolition for bricks. On April 22, 1930, a decision was made to demolish the Blagoveshchensky Cathedral, and at that time, the monastery bell tower was also destroyed.

In 1949, a large part of the monastery was transferred to the Astrakhan Regional Military Commissariat, which is still located there. In the one-story wooden cells in the northern and eastern parts of the convent, there is now private residential housing. The chapel of St. Nicholas was long under an apartment but has survived, as has most of the monastery wall, although its condition leaves much to be desired. At the walls of the monastery on Kalinina Street, a cross has been erected in honor of the holy saints Methodius and Cyril.