Next to the Peter and Paul Cathedral rises the Holy Trinity Women's Monastery, which became a convent in early 2003, previously serving as a cathedral.

Odessa Street, where the monastery is located, was named in 1946. Before that, since 1839, it was known as Greek Street, which was justified as Greeks lived here. From the 18th century, trading rows with columns under a tiled roof have been preserved.

The first wooden Greek church was built in 1796. Since 1826, the Holy Trinity Church housed a Greek gymnasium. In 1868, the old church was dismantled, and a new building was constructed according to the design of architect I.F. Kolodin. The temple has a cross-shaped plan with an octagonal light drum and a low bell tower. Light penetrates through arched windows, and the walls are decorated with ornamental elements. The domes are painted blue and topped with ornate crosses. Recently, a new baptistery was built in the monastery.

The interior decoration of the temple is also impressive: the sails depict the four evangelists, and in the dome, the image of the Lord. The cathedral has two chapels: one dedicated to St. Nicholas of Myra, the other to the holy equal-to-the-apostles Constantine and Helen. The temple is consecrated in honor of the Holy Trinity.

In the 1930s, priests who had performed the feat of martyrs served in the Holy Trinity Cathedral. In 1935 and 1937, Bishop Porphyrius Gulievich served here, who led the Crimean diocese during a difficult time for the Church. He attracted faithful clergymen and fought against the Renovationists. The bishop was humble and meek, especially in prayer. He endured arrests and exiles with courage. In 1937, he was arrested in Kazakhstan and sentenced to death.

Archpriest Nikolai Mezentsev, the closest assistant to the bishop, was born in the Oryol province. He lost his parents early and received a spiritual education at state expense. In 1891, he arrived in Simferopol and became a teacher at the Taurida Theological Seminary. In 1903, he became the rector of the Nativity of the Mother of God Church. After the establishment of Soviet power, he was arrested for attempting to preserve church values. In 1923, he was sentenced to three years in a concentration camp but was released early. Later, he served in the Peter and Paul Cathedral and the Holy Trinity Church. In 1933, he was arrested again. In 1937, he was arrested again and sentenced to death. In 1997, he and Bishop Porphyrius were canonized as local saints.

The relics of Saint Luke, an outstanding physician and priest, rest in the monastery. Valentin Voyno-Yasenetsky was born in 1877 in Kerch. He studied at Kyiv University in the medical faculty. In 1904, he went to the Far East, where he successfully began his surgical practice. In 1915, his book "Regional Anesthesia" was published. In 1923, he became a priest and monk, taking the name Luke. He was arrested three times and exiled. In 1942, he became an archbishop. In 1946, he arrived in Crimea, where he actively worked on restoring the diocese. He died in 1961, and his relics were transferred to the Holy Trinity Cathedral in 1996. He left a rich scientific and spiritual legacy.

The cathedral houses the icon of the Mother of God "Sorrowful," which was renewed in 1998. It has become a holy relic for all of Crimea and attracts pilgrims. In 1999, the icon was taken around the entire peninsula in a procession. It is now located in the monastery, for which a beautiful cover has been made. The veneration of the icon is great, and people come from afar to pay their respects.