The stone Krestovozdvizhensky church with one altar was built in 1880 on a plot of more than 4000 sq. m., which was purchased by the Yekaterinburg merchant and city mayor V.V. Krivtsov. The church served as a home church for the Children's Shelter of the Yekaterinburg Charity Society. Until the summer of 1919, it functioned as a regimental church for the regiments of the Yekaterinburg garrison, located in the Orovai barracks, where a military unit is still based today. There was a choir at the church, consisting of 15 boys from the shelter, two clerks from the Yekaterinburg district military command, an assistant supervisor of the military hospital, and soldiers from the Yekaterinburg local team. From 1927 to 1930, the church, remaining loyal to Metropolitan Sergius, was the cathedral of Yekaterinburg, and 12 priests served there, which is why it was called the "Cathedral of the Twelve Apostles."

In 1930, the Bolshevik authorities closed the church and carried out destructive renovations, removing the dome and bell tower. The church community was merged with the community of the Alexander Nevsky Cathedral of the former Novo-Tikhvin Women's Monastery, which by that time had already been closed.

After the cessation of services, the Krestovozdvizhensky church was used as a cinema. During the war, an elephant from an evacuated zoo lived in the altar of the church. After the war, the building was divided into two floors: the first housed workshops of the Artistic Fund, while the second accommodated various architectural organizations. In the summer, plaster busts of Lenin were dried around the church.

In the early 1990s, the authorities planned to sell the building to a commercial organization that promised to restore its appearance and use it for trading goods from Turkey. Upon learning of this, the local Cossacks appealed to the authorities to transfer the church to the Russian Orthodox Church. Ultimately, the plans changed, and the building was decided to be used for a recording studio of the Sverdlovsk State Television and Radio Broadcasting Company. However, due to funding cuts in 1993, the church was handed over to the Yekaterinburg Diocese. The Cossack community monitored the preservation of the remaining items in the church. The first rector was priest Andrei Dedyukhin-Sitsky, a hereditary Cossack.

Services were held on the second floor in the former bell tower, where the monastery refectory is now located. A temporary altar was consecrated in honor of Saint George the Victorious. In cold weather, heating was provided by an electric heat gun.

During the Soviet era, a red-brick extension was built on the site of the former porch, which housed the Cossack headquarters. After a fire destroyed the altar of Saint George, Bishop Nikon Mironov ordered the cessation of Cossack activities at the church.

In the fall of 1994, a procession took place, transferring the Iveron Icon of the Mother of God with the relics of saints from the John the Baptist Cathedral to the church.

On December 26, 1995, the Holy Synod, at the request of Bishop Nikon Mironov, decided to establish a monastery at the church. Currently, three hieromonks and one hierodeacon live in the monastery, as well as Christian youth students. The residents participate in the activities of the missionary department of the diocese, as the monastery serves as a missionary center for the Central Deanery. It conducts anti-cult activities and works with youth, many of whom study at the Yekaterinburg Orthodox Theological Seminary.

The relics of the monastery church include a particle of the Honorable and Life-Giving Cross of the Lord, the Iveron Icon of the Mother of God with the relics of Saints Mitrophan of Voronezh and Tikhon of Zadonsk, Saint Righteous Simeon of Verkhoturye, Saints Venerable Dalmat of Iset and Arefa of Verkhoturye, Blessed Cosmas of Verkhoturye, as well as icons of Venerable Seraphim of Sarov, Saint Luke of Simferopol, and Holy Martyr Faddeus, Archbishop of Tver, and revered icons of the Mother of God of Tikhvin and Saint Nicholas the Wonderworker.