In the summer of 1837, one of the best houses in the city was purchased for 72 thousand rubles to house the school. This house, owned by merchant Y. I. Bilibilin, was located on Arkhangelsky Lane, now known as Darwin Street.

For three years, until September 1840, renovations were carried out in the building for the needs of the school, and only after that did classes begin.

In 1903, at a congress of the diocesan clergy, it was decided to build a church at the spiritual school. On December 1, 1904, the church was consecrated in honor of St. Guriy of Kazan.

The school ceased operations in 1918.

In 1992, the Kaluga Spiritual School was reopened. The teaching staff was formed from representatives of the clergy of the diocese. The curriculum was modeled after Moscow spiritual schools and included a three-year course.

On August 3, 1995, the building of the former spiritual school (Darwin St., 13/33) was transferred to the diocese. Until that time, it housed the Kaluga Medical and Technical Laser Center, a small business support agency, a sewing workshop, a pawnshop, an economic school, and other organizations.

From August 1995, the Kazan Girls' Monastery was located in the building of the spiritual school. In 2011, the monastery moved to restored buildings in the village of Spas.

In 1996, the school was transformed into a seminary with a four-year course of study.

By the decree of the Holy Synod on July 7, 2002, the Kaluga Women's Diocesan School was transformed into the Kaluga Spiritual School with the admission of students of both genders, and it was officially opened on August 31, 2002.