Since 1999, the Presentation Church has been affiliated with the monastery, located behind its fence. Built in 1780, it is considered one of the most beautiful in the city and is a monument of historical and cultural significance of the federal level from the 18th century.

In the spring of 1700, residents of the Uspenskaya Church parish, where shipbuilding was taking place, moved to the Akatov suburb: Grigory Koshkin, Zakhar Eliseev, and Yakov Anikeev. In October of the same year, they appealed to Peter I, who was in Voronezh, for permission to cut down trees for the construction of a church. The church was soon built and consecrated by Bishop Mitrofan in the name of the Presentation of the Blessed Virgin Mary.

The appearance of the small wooden church is unknown. The fire of 1748 did not affect it. In 1770, due to its dilapidation and small capacity, it was replaced by a stone church with two altars. On November 10, 1771, an altar in the name of Saint John the Warrior was consecrated.

The main church was fully completed by June 1780. Later, in the mid-19th century, another altar in the refectory was added in the name of Saints Mitrofan of Voronezh and Tikhon of Zadonsk. The church did not undergo significant alterations. In a historical description published in "Voronezh Antiquity" in 1908, priest Nikolai Nikonov detailed its appearance, even noting its dimensions: the dome had a height of 27 arshins (19 meters).

The heads of the church and bell tower were covered in gold and adorned with gilded crosses. The surroundings were filled with the ringing of nine bells, ranging from 162 poods (1.6 tons) to 17 pounds (7 kg). Three particularly revered icons in silver settings were mentioned: the icon of the Mother of God "Joy of All Who Sorrow," and the icons of Saints Sisinnius of Laodicea and John the Warrior. Among rare items was a chalice with the inscription: "On the 15th day of November 1704, these vessels were given to the holy church for the Presentation of the Most Holy Theotokos by Grigory Danilov son Koshkin, a townsman of Voronezh, from his parents; weighing a total of 371 zolotniks." Merchant Anikeev donated a Gospel published in 1698 to the church.

Since 1887, there was a guardianship for less affluent parishioners at the Presentation Church, and from 1891, the first women's parish school with handicraft classes in the diocese. Wealthy merchants from nearby families were buried in the churchyard for a long time.

In this church, the great Russian writer Ivan Bunin and the outstanding artist Nikolai Ge, known for his historical and religious paintings, were baptized.

In the early 1920s, the church voluntarily parted with the few valuables it had. Liturgical items went to state storage. The closure of the Presentation Church occurred in the mid-1930s. For some time, the building stood empty, and before the war, it was occupied by a craft cooperative. In 1943, when people returned to the partially destroyed city, the church was used for housing. Rooms appeared in the altar and refectory. The next owner of the church became the city repair and construction management. In the mid-1970s, recalling the former beauty of the Presentation Church, it was decided to restore it and open an archaeological branch of the Local History Museum. A decade-long restoration did not bring significant changes to the appearance of the building. In 1990, the Presentation Church was returned to the diocese in a deplorable state.

In 1994, the reconstruction of the building's exterior was completed, with work carried out by stonemason-restorers under the guidance of Igor Burov. Architectural details were highlighted in color. The adjacent territory was improved, and the churchyard regained its fence. The interior finishing of the church lasted another five years. After a lengthy restoration, the Presentation Church was consecrated on December 4, 1999, and was affiliated with the Alekseevsky Akatov Monastery.

Currently, the Presentation Church is under restoration, with frescoes being painted.