In 1785, in the village of Glushkovo, the owner of a manufactory, Kozma Matveev, built a church dedicated to the Holy Trinity. In the first quarter of the 19th century, to commemorate the decade of victory over Napoleon, Count Sergey Petrovich Potemkin financed the reconstruction of this church. In 1895, he was buried in the church's crypt.

In the 20th century, the Church faced difficult times: many churches and monasteries were destroyed and looted, and sacred items were destroyed. The Holy Trinity Church also suffered: in 1933, the bell tower was destroyed, church utensils were looted, the interior decoration was destroyed, and the crosses and dome were removed. Only the two-meter walls and six columns at the entrance survived. Currently, the former church houses the district cultural center.

Church life in the Holy Trinity parish resumed in 1959 with the opening of a prayer house. In 1991, after three decades, Archpriest Alexander Postnikov began the construction of a new stone church twenty meters from the site where the Church of the Holy Apostles Peter and Paul, built in 1848 by S. P. Potemkin and destroyed in the 1930s, once stood. The church was built with donations from organizations in the Glushkovsky district, parishioners, and local residents.

In 1996, on Easter, the first service was held in the new, still unrenovated church. In 1999, on the feast of the Meeting of the Vladimir Icon of the Mother of God (September 8), the new church was solemnly consecrated in honor of the Life-Giving Trinity by Archbishop Juvenaly (Tarasov) of Kursk and Rylsk. In 2002, a four-tiered iconostasis was installed and consecrated in the church. In 2006, the parish celebrated the tenth anniversary of the church.