The Akhtyrsky Women's Monastery was founded by priest Ioann Levitsky from the village of Lopukhovka in the Kamyshin district of Saratov province and peasant Illarion Yakovlevich Dvoychenk from Tambov. The latter, while traveling to holy places, spent many years in the Verkola Monastery in Arkhangelsk province and ultimately secretly took monastic vows on Mount Athos, adopting the name Nikodim. As was customary in the East, he was sent to Russia to collect donations and establish a monastery in honor of the Most Holy Theotokos. In the summer of 1865, he met with Father Ioann Levitsky in Lopukhovka and convinced him to create a community there.

In 1860, 30 sisters wishing to serve God gathered in Kamyshin and organized a communal household, which included a house with a refectory, a bathhouse, a barn, and a cellar. The first abbess was nun Maria. However, the community did not continue to develop in the city, and it was decided to carry on the work away from the hustle and bustle of urban life.

In 1871, Father Ioann and Father Nikodim purchased 281 desyatinas of land near the settlement of Gusevka, now in the Olkhovsky district of Volgograd region. According to the first settlers, even before the foundation of the community, miracles occurred here: invisible bells rang, prayers were heard, and light appeared as from candles. The founders also acquired three estate plots in the settlement and the house of landowner Nikolev for the sisters. By 1874, the 'sixty' and 'five hundred' plots and 'plantation' were purchased. In total, Father Nikodim transferred 1708 desyatinas of land to the community, along with a windmill and other buildings. Local peasants helped with small but sincere donations.

From 1929, the church was abandoned and used by the Gusevsky village council as a grain storage facility. In 1934, the commission on cult issues of the Stalingrad regional executive committee decided to satisfy the request of the presidium of the Olkhovsky district executive committee and close the church, repurposing it for a cultural and educational institution. The parish Holy Trinity Church, built in 1837, was also closed on September 22, 1934, and dismantled for building materials for a new bathhouse, with a garden planted in its place. In 1945, according to reports from local authorities, in the Akhtyrsky Monastery 'there is no church building, there is a former church house occupied by an incomplete secondary school, and other small buildings belonging to the NSSH are planned for a children's home.'

The process of returning the monastic property, which was used by the secondary school and the collective farm, dragged on and only began to move forward with the arrival of Archbishop German Timofeev of Volgograd and Kamyshin in Gusevka in July 1994.

The Akhtyrsky Women's Monastery of the Mother of God in the village of Gusevka, Olkhovsky district of Volgograd, opened on February 21, 1996. The residents had to acquire housing to relocate several families living in the old mansion. Local believers collected the surviving monastery icons from their homes. Since the return of the monastery, the house for the abbess and sisters, the old refectory for administrative purposes, and the remaining farm buildings have been renovated, a new refectory, bathhouse, warehouses have been built, and an icon shop and prosphora have been equipped. Restoration of the old cell building is underway.

On October 10, 2001, the first stone was laid for the foundation of a stone church in honor of the Akhtyrskaya Icon of the Mother of God. The construction of the church has been completed. In the summer of 2004, an old well was found, over which a chapel was built.