The Church of the Sign of the Mother of God and the Holy Myrrhbearers is the first stone parish church in the Upper Posad of Nizhny Novgorod. It is the first church in the history of ancient Russian architecture of the "ship" type, where the altar, prayer hall, refectory, and bell tower above the western entrance are strictly aligned along the axis. In this parish, the miracle workers Euphemius of Suzdal and Macarius of Zheltovodsk were born. Two churches are located in one building: the winter church, dedicated to the icon of the Mother of God "The Sign," and the summer church of the Myrrhbearers.

Originally, a wooden church was built here in the 13th century, and in 1649, a two-story stone church was constructed. In 1805, with funds from merchant P. Perepletchikov, a two-story brick chapel was added to the northern side of the refectory: downstairs in honor of the apostles Peter and Paul, upstairs, of Euphemius of Suzdal, and in the refectory, of Macarius of Zheltovodsk. After the fire in 1848, the frescoes of the interiors were restored. In 1894 and 1896, the chapels were expanded. M.P. Dmitriev took historically valuable photographs that were used in the restoration of the church in the early 21st century.

After the revolution, the church was closed, the domes were destroyed, and the building housed a technical school for light industry. In the 1990s, the church was returned to the Nizhny Novgorod diocese. By July 2004, a dome was installed over the chapel of the Venerable Macarius of Zheltovodsk and the Unzhensky Miracle Worker, and work was underway to gild the main dome. The gilding of two crosses was carried out at the Arzamas residence of the Holy Trinity Serafimo-Diveevsky Monastery. In September 2004, Bishop Georgy consecrated the new domes and crosses, and on October 10, 2006, a large cross was consecrated and installed on the bell tower.

Next to the church, there is a city tram ring. In the summer of 2005, a 200-meter section of the tram track was replaced using vibration-insulating material.

In 2007, Bishop Georgy, visiting the church, instructed to prepare an estimate and develop a project for the reconstruction of the lower church.

In March 2008, the government of the Nizhny Novgorod region approved the boundaries of the territories of cultural heritage sites of federal significance, including the church of the Myrrhbearers and the House of Peter the Great.

On December 31, 2009, Archbishop Georgy conducted the Divine Liturgy. After the service, the rector of the church, Archimandrite Vasily Danilov, presented the bishop with an icon of the Savior. In April 2011, the bishop noted the completion of the first stage of restoration, emphasizing that the church was externally improved, and work on the interior decoration and the arrangement of iconostases in the four chapels was forthcoming. A project album was prepared for each iconostasis. In July, work began on the iconostasis for the lower chapel in honor of the icon of the Mother of God "The Sign." The wooden iconostasis will be two-tiered and made in the Byzantine style by the staff of the Ark workshop. In September, the painting of the altar in the lower chapel began.

On January 25, 2012, Archbishop Georgy performed the rite of the Great Consecration of the lower church in honor of the icon of the Mother of God "The Sign." Then the bishop served the first Divine Liturgy in many years. On December 29, the chapel in honor of the Venerables Macarius of Zheltovodsk and Euphemius of Suzdal was consecrated.

On December 18, 2013, Metropolitan Georgy, with the co-serving Bishop Siluan of Lyskovsky and Lukoyanovsky, performed the rite of the Great Consecration of the chapel in honor of the holy apostles Peter and Paul.