The Ascension Church is located in the southwest of Vladimir, on a hill that descends to the Klyazma River, beyond the city ramparts. In the late 12th century, there was a monastery of the same name on the site of the current church, mentioned in chronicles from 1187 and 1218. However, apart from these mentions, there are no other historical records about the monastery, its condition, or its fate. It was likely destroyed during the Tatar invasion in 1237, as it was outside the city fortifications and on the path of the Tatars' advance.

When exactly a new church was built on the site of the destroyed one is unknown, but in the 17th century, it is mentioned in the list of Vladimir churches. In the patriarchal books from 1628, it is listed as 'the Church of the Ascension of Christ on Ascension Hill.' In the documents of the patriarchal treasury office from 1652, this church is referred to as a 'gun church,' indicating the presence of a monastery in the past. There was another warm church dedicated to the Protection of the Holy Virgin, built in the early 17th century, but around the middle of the century, it burned down, and a new one was erected in 1652 at a different location. In 1718, priest Andrei Grigoriev appealed to the treasury patriarchal office with a request to build a new stone church, as the wooden structures had become dilapidated. The decree was issued, and by the end of the 18th century, a stone church was built in place of the two wooden ones. The year of construction is specified in the 'Topographical Description of Provincial Vladimir 1761,' which states that the church with the chapel of the Annunciation of the Holy Virgin was built in 1724. This stone temple has survived to this day, and it has a stone bell tower. The church has three altars: the main one in honor of the Ascension of the Lord, a cold chapel in honor of the Protection of the Holy Virgin, and a warm one in honor of the Annunciation of the Holy Virgin. The icons in the iconostasis of the cold church, created in 1812, are made in the Italian style. Church documents, including copies of metric books from 1780 and confession lists from 1821, are preserved intact. The church has a capital of 4725 rubles, the interest from which is used. The land plot belonging to the church consists of about 2 desyatinas of estate land and 35 desyatinas of haymaking land. Plans and boundary books for the land are also preserved. According to the clergy's staff, there is a priest and a psalmist.