In 1731, the construction of the stone cathedral of the Assumption of the Mother of God began, with chapels dedicated to the Beheading of John the Baptist and Saint Nicholas, but the chapels were not completed at that time.

In 1861, the refectory part of the church was dismantled and rebuilt, expanding on both sides to accommodate two chapels. On the right, a chapel was created in honor of the Protection of the Blessed Virgin Mary, and on the left, the iconostasis from the old Nicholas church was moved and a chapel in honor of Saint Nicholas was arranged.

In 1994, after the monastery was returned to the Church, the Assumption church was the first to be restored, remaining the only link to the past, as the rest had been destroyed. In connection with the construction of the building with the Nicholas church, on October 14, 2001, the right chapel was consecrated in honor of the Protection of the Mother of God, and on September 13, 2003, the central Assumption chapel and the left chapel of John the Baptist were consecrated.

The Church of the Assumption of the Blessed Virgin Mary, located in the center of the monastery, is considered the main one. Inside, there are three reliquaries containing parts of relics. The Assumption chapel houses the main relics: an icon of Saint Nicholas with a part of his relics, a shoe of Saint Spyridon of Trimythous, an icon of the Pochaev Mother of God with parts of the relics of Job and Amphilochius of Pochaev, parts of the relics of Prince Vladimir and Princess Olga, as well as a reliquary with parts of the relics of the Glinsk elders.

Another reliquary is located in the niche of the right, Protection chapel, where parts of the relics of saints such as Luke of Crimea, Innocent of Moscow, Philaret of Moscow, Ambrose of Mediolanum, and others are kept. Here, the relics of the righteous princes Peter and Fevronia of Murom, as well as other saints and martyrs, are also found.

The third reliquary is located in the niche of the left chapel of the Beheading of John the Baptist and contains parts of the relics of the venerable Gerasim of Jordan, Moses the Ugrian of the Caves, Jonah of Kiev, and many other saints.