The village is home to the largest church of the Nativity of the Blessed Virgin Mary in the Volga region. This magnificent three-altar stone church was built in the late 19th and early 20th centuries and consecrated in 1899. The architect was Sergey Karyagin, known for his works in Moscow and St. Petersburg.

The current church is the third one in the village. Two others existed before it. The first, a wooden church, was consecrated in honor of St. Nicholas the Wonderworker. Its icon was carried around the village, blessing it, which gave the village its name, Nikolskoye. Due to the changing course of the Volga River, the land under the church collapsed, and it fell into the river.

The second church, originally intended to be a two-altar church in honor of the Blessed Virgin Mary and St. Nicholas the Wonderworker, was also destroyed by the river. Therefore, the third church was decided to be built away from the shore. At a village meeting, it was decided to make it again a two-altar church, but fishermen suggested adding a third altar in honor of the Holy Apostles Peter and Paul, the patrons of fishermen.

During the war, the church was closed, and services were held in the basements, where residents sheltered from air raids. A crack remains on the bell tower from those times, caused by bombings, but it does not increase.

The Soviet authorities planned to blow up the church, but the chairman of the village council, Pavel Iosifovich Kidebu, being a believer, proposed to use it as a grain storage facility. He understood the risk but defended the church and did not allow the bells to be removed. However, he was later executed on false charges, and the bells were taken down after all.

After the war, services resumed in the church itself, aided by a letter from I. Stalin, which eased pressure on the church and recognized the right of believers to freedom of worship.

The restoration of the church in Nikolskoye is associated with Archpriest Nikolai Sitkin, who held services in the basement during the war.