The Annunciation Church, built in 1515, is one of the oldest structures of the Protection Monastery alongside the bell tower and cathedral. This building is a unique example of ancient Russian architecture, combining features of a fortress tower and a church. The two-storey rectangular tower is crowned with a miniature church reminiscent of large cathedrals. Inside, there is a central area measuring 5x4 meters, an altar, and galleries leading to prospective portals. In the southeast part, there is a room for a separate altar, so small that it barely accommodates two people. According to legend, this was the prayer room of Grand Duchess Solomonia, who was exiled to the monastery under the name Sophia.

In the northeast part, there is a square room, separated from the central altar, which was previously connected to the gallery. This is a separate chapel with an altar in honor of Archangel Michael. During the stay of Evdokia Lopukhina in the monastery, the chapel was altered and turned into a prayer room. Here stood a chair adorned with a coat of arms and covered with dark green cloth, which can now be seen in the local museum. The museum also displays a chandelier made of Venetian crystal with a gilded inscription "Tsaritsa," which previously hung from the ceiling.

Due to the arrangement of the prayer room, the interior of the church was altered: the corner domes of the chapels were dismantled, the arcades of the gallery were bricked up, and the gallery itself was divided into separate rooms. In the western part of the arch, a door was created connecting the church with the cell of nun Elena, the exiled queen, through wooden passages. The gallery had chimneys punched through for heating the rooms where Moscow's secret messengers were located.

The southern facade of the church is adorned with rolls, a cornice, and strips reminiscent of folk wood carving. This is one of the first examples of using elements of folk art in the architecture of Suzdal.

The Annunciation Church is the work of local craftsmen. It is located on the southern side of the walls of the Protection Monastery and faces Moscow, on the main road along which guests from the capital arrived in Suzdal. This road was also traveled by Ivan the Terrible for the celebration of the Protection and to pay homage to his ancestors, bringing with him precious gifts that replenished the monastery's rich treasury.

Today, the church has been restored to its original appearance according to the design of architect A. Varganov.