In 1826, a community of female hermits was established in the village of Betki in the Menzelinsk district. Six years later, it significantly expanded, and the elder sister Stefania Bychkova, who became Mother Filareta in monasticism, appealed to Bishop Arkady Fyodorov for patronage and intercession before the Holy Synod to transform the community into a monastery, which was planned to be located in Ufa. Blessing was received from Abbot Filaret of the Glinsk Monastery, who drafted the community's charter. In 1833, the Synod issued a decree requiring proposals for the future monastery's means of existence. Stefania Stepanova, who lived in Ufa at the time, purchased several houses on Usolskaya Street, where the monastery was to be built, providing 3480 rubles for this purpose.

In 1838, at the request of Mother Filareta and the presentation of Bishop Ioannikiya, the community was renamed Blagoveshchensky Women's Monastery. The monastery was located on the site of the old Uspensky Men's Monastery in Ufa, which existed from 1595 to 1764. Since its opening, the monastery received a subsidy from the treasury of 300 rubles and 150 desyatins of meadow land across the Ufa River. Yakov Pavlovich Barsov donated 400 desyatins of land and a mill on the Biry River to the monastery.

The monastery was managed by the abbess and was not staffed. The women living there were not subjected to the rite of tonsure but had to obey the decisions of the superior. Life in the monastery included daily prayers and detachment from the outside world. Initially, the monastery consisted of a two-story wooden house, a guesthouse, and other service premises. As the number of sisters increased, so did the prosperity of the monastery.

The monastery was surrounded by a stone wall with four towers, each topped with a conical roof. The total length of the walls was about 800 meters. The main gate was located on Usolskaya Street, while the eastern gate was under a stone arch. The southern gate opened onto the Belaya River. The monastery had a triangular shape with a cut-off corner, bordered by Usolskaya Street on the north and the embankment of the Belaya River on the south.

Initially, the monastery owned an old wooden church with a bell tower, the origin and location of which are unknown. From 1850 to 1852, the first stone church in honor of Saint Prince Alexander Nevsky was built. In 1850, the construction of a summer cathedral church in honor of the Annunciation of the Blessed Virgin Mary began, which was consecrated in 1867. In 1897, a third brick church in honor of the Iveron Icon of the Mother of God was consecrated.

The monastery possessed valuable items of worship, including a silver cross and a Gospel from 1763. There were more than 20 different buildings on the territory, including a candle factory, workshops, and utility buildings. The monastery owned eight houses in Ufa, including a large brick building at the corner of Uspenskaya and Alexandrovskaya Streets.

By the early 20th century, up to 600 sisters lived in the monastery. The monastery engaged in charity, opening schools and dining halls for the needy. In the early years of Soviet power, the monastery was closed, and Abbess Klavdiya was executed. In 1922, church valuables were confiscated. In 1929, the monastery churches were closed, and in 1936, the monastery buildings housed a pharmacy warehouse. Subsequently, there were a hospital, morgue, and other institutions.

As of now, three of the 30 buildings of the monastery have survived: the buildings of the Iveron and Alexander Nevsky churches, the sisters' dormitory, and the monastery school. All of them require restoration to restore the historical appearance of the monastery.