The Orthodox church of the Chelyabinsk diocese of the Russian Orthodox Church is the largest in the city. The first Holy Trinity church was founded in Zarechye in 1768. In 1909, it was decided to build a new church on this site, and construction began in 1911. The church was consecrated on October 12, 1914. During the Soviet era, on October 1, 1929, the church was closed, and there were plans to open a cinema in its building, which would have been the first in the Zarechye part of the city. However, the city authorities decided to place exhibits of the local history museum there. From 1931 to 1932, the museum was moved to the Christ the Savior Cathedral, but after its demolition, it returned to the building of the Holy Trinity church. In 1987-1988, the building was restored, and in 1990 it was transferred to the Chelyabinsk and Zlatoust diocese and consecrated again. The interior artistic works were done by artist N. F. Kostyuk.

The Central Historical Archive of Moscow reported that, besides the insurance assessment, no other documents about the Holy Trinity Cathedral of Chelyabinsk have been found.

The cathedral is adorned with rich frescoes in the style of Vasnetsov and a beautiful cedar iconostasis. The church choir performs rare and solemn works by composers such as Rachmaninoff, Bortnyansky, Chesnokov, Tchaikovsky, and others.

After the destruction of the Christ the Savior Cathedral, the Holy Trinity church became the largest temple in Chelyabinsk. The church houses relics of saints: St. Seraphim of Sarov, St. Trifon the Great Martyr, St. Andrew the First-Called Apostle, St. Panteleimon the Great Martyr, and St. Matrona of Moscow.