Throughout its existence, the Ryazan Kremlin remained wooden. In the 16th century, the first stone tower, Glebovskaya, was built on the site of the modern Cathedral Bell Tower. The city walls were constructed from durable oak and surrounded the entire Kremlin hill. The upper part of the walls ended with a "break", a covered platform made of planks that protruded outward, making assaults difficult. On this platform were the city's defenders and sentinels, who could move freely along the wall from one tower to another. The walls supported 12 towers, some of which have not survived in name.

The Glebovskaya tower, with the Glebov gates, got its name due to its location: it faced the small fortress of Borisov-Glebov, the residence of the Ryazan bishops. The tower was made of stone and had an open area with a milestone bell weighing 11 poods. On the second floor was a chapel above the gate with an icon of the Mother of God Odigitria, which was later transferred to the Ilyinsky church.

The Spasskaya tower received its name from the Unmade Image of the Savior, which was later moved to the Spassky Cathedral on the Yar.

The Secret Tower with the Secret Gates had a secret passage used to draw water from the Trubezh during sieges.

The Dukhovskaya tower was located on the territory of the Dukhovsky Monastery.

The Ipatskaya tower with the Ipatsky gates, the Vsesvyatskaya tower, and the Ryazanskaya tower with the Ryazanskie gates, which faced the "Ryazan side", at the foot of which began the Great Ryazan tract leading to Ryazan, were also part of the fortification system.

The Vvedenskaya tower completed the list. By the 18th century, the wooden walls had deteriorated and were demolished after the approval of Catherine's urban planning project after 1778.