The monastery has long had a wooden Trinity church. Researchers suggest that it may have been damaged during the fire on September 3, 1647, when a significant part of Pereyaslavl Ryazansky was destroyed by fire. In 1685, a stone church was built on this site with an altar dedicated to St. Alexius of Moscow. After the monastery was closed in 1919, the church continued to function as a parish church. From 1929, the building was used for grain storage. In the 1950s, workshops of a local automotive equipment factory were located in the church, bell tower, and northern tower.

The church was consecrated on June 16, 2000. It is made of brick, plastered, and covered with iron, designed in the style of Russian Baroque and belongs to the type "octagon on a square." The transition to the octagon is achieved with stepped trompes. The composition of the building is very simple: the main longitudinal volume includes a three-light church and two-light refectory and altar. The light octagon is topped with a dome with eight segments, which is finished with a blind faceted lantern with an onion-shaped top. During the Soviet era, factory buildings were added to the west and north sides of the structure, significantly distorting its external and internal appearance.