The Pokrovsky Monastery in Kiev is an Orthodox women's monastery in the Kiev diocese of the Ukrainian Orthodox Church. It is also known as the Kiev-Pokrovsky Monastery or the Monastery of the Protection of the Mother of God. Originally named the Kiev Pokrovsky Women's Dormitory Monastery, it was founded on January 11, 1889, by Grand Duchess Alexandra Petrovna Romanova, the wife of Grand Duke Nikolai Nikolaevich the Elder. Before the construction of the monastery, there was a house church on this site where, according to legend, Alexandra Petrovna was healed from illness while praying before the icon of the Mother of God of Pochaev. Later, she took monastic vows under the name Anastasia and became the abbess.

In 1881, Alexandra Petrovna left St. Petersburg and moved to Kiev, where she began to realize her idea of creating a monastic community. She envisioned it not only as a women's monastery but also as a medical institution for the poor, where nuns and novices would perform the duties of junior medical staff. The Grand Duchess donated all her funds to establish the monastery, and part of the funds was also provided by her family. Over 20 years, about 30 buildings were constructed on the monastery grounds, including churches, a school, dormitories for the sisters, workshops, a hotel, as well as medical facilities such as a free hospital with therapeutic and surgical departments, a shelter for the blind and infirm, an outpatient clinic, and a pharmacy. The chief physician of the hospital was Nikolai Viktorovich Solomka, who helped the Grand Duchess equip the hospitals with modern equipment. It was here that the first X-ray machine in Kiev and Russia appeared. In the first decade of the hospital's operation, 5,020 patients were treated, over five million medications were dispensed, and 2,298 surgeries were performed. Alexandra Petrovna often assisted the surgeons. At the end of the 19th century, new hospital and clinic buildings were constructed with funds from the royal family, allowing the number of patients accepted to increase to 500 per day. Alexandra Petrovna passed away in the monastery on April 13, 1900, but her plans continued to be realized.

The design and construction of the monastery were carried out by Vladimir Nikolaevich Nikolaev, the diocesan architect of Kiev. The Pokrov Church and the Nikolsky Cathedral, designed by the Grand Duchess's son, Peter Nikolaevich, were built in the neo-Russian style. The foundation of the Nikolsky Cathedral was laid on August 21, 1896, with the first stone laid by Emperor Nicholas II, the second by Empress Alexandra Fyodorovna, and the third by Grand Duchess Alexandra Petrovna. The cathedral was built with funds from the Kiev benefactor Nikola Artemievich Tereshchenko and took 15 years to complete. On May 9, 1911, Bishop Pavel of Chigirin consecrated the main altar of the cathedral. The Nikolsky Cathedral now has two floors and six altars.

In 1925, the monastery was closed, and the cathedral's domes were demolished. It was reopened in the autumn of 1941 after German troops occupied Kiev. This was aided by nun Epistimia and Archbishop Antoniy. In December 1941, Bishop Panteleimon of Lviv established a temporary residence here. In 1943, as the front approached, the sisters of the monastery refused evacuation and remained in a besieged position in the lower church of the cathedral for 40 days.

From 1943 to 1945, a hospital operated on the monastery grounds, and from 1945 to 1948, a medical unit. The committee for the cathedral's restoration was headed by the sister of Patriarch Alexy, nun Eupraxia. The interior renovation was completed by May 1949, when the main altar was consecrated. However, the cathedral remained without domes on the outside. In 1981, it was damaged by a fire caused by a lightning strike. Water used to extinguish the fire damaged the plaster, but the walls and vaults were painted. In the 1990s, the wooden Pokrovsky Church was rebuilt in stone and painted, and its domes were restored. The consecration of the church took place in May 1999, and the cathedral regained its original appearance in 2006 and 2010.