The height of the church is 30.5 meters. It is a single-story building but is situated on a high basement. The church has one dome, with a five-sided altar apse, topped with an octagonal blind drum, a neck, a dome, and a cross.

Previously, there was a stone porch supported by pillars on three sides of the building, which was dismantled in 1792 due to its dilapidation. Now, only a fragment in the form of a staircase on the southern side of the church remains as a reminder of it. In the basement, there are two chambers, one of which serves as the burial place for the abbots of the monastery. The church had two entrances: one on the southern side through a covered porch on arched brick pillars, and the other from the abbot's chambers.

The church suffered greatly during the Patriotic War of 1812 and was restored and consecrated only in 1819. In 1972, during the repair of the church's dome, an unexploded shell from the Great Patriotic War was discovered under the roof. Retreating fascist troops shelled the area, and one of the shells hit the church's dome but did not explode. The shell lay in the church's roof for thirty years, and it was necessary to call in sappers to defuse it. At that time, during the repairs, the upper part of the church was reinforced, the roof was replaced, the wooden staircase was redone, and the foundation on the northern side was strengthened.

On July 27, 1992, the Church of Mitrophan was consecrated, the relics of the Martyrs of Chozhevitsy were placed in the altar, and regular services began. In 2011, the old galvanized roof was replaced with a copper one, and the building was plastered and painted on the outside.