The Ankushino settlement was first mentioned in 1781 as part of the parish of the village of Zagarye, where 10 people lived. The village was located on a busy road, and during winter fairs and holidays, residents from Zagarye, Sovya, Bobino, Vyatka, and Slobodskoye would come here.

By 1860, the Zagarsky parish had significantly grown, and peasants from nearby villages connected to Ankushino petitioned for the establishment of a separate parish. On September 15, 1860, the Vyatka bishop Agafangel allowed the founding of a new village in Ankushino called "Krestovozdvizhenskoe," named after the church, with one priest and two deacons.

The location for the new village was well chosen: fertile lands, beautiful meadows, and three springs with clean drinking water. These springs gave the village a new name, "Trekhklyuchinskoe," under which it began to be mentioned in church documents.

By the end of 1860, a new Ankushino parish was formed from the villages of the Zagarsky village. Initially, a wooden prayer house was built in the village, which was soon rebuilt into a wooden church. The small Krestovozdvizhenskaya church was consecrated on March 19, 1861.

In 1879, local craftsmen began the construction of a stone church funded by the peasants. The stone masonry was completed in 1884, followed by plastering and interior work. On November 10, 1886, the new stone church in the village of Ankushino-Trekhklyuchinskoe was consecrated. In 1896, the expansion of the church by one altar began, which was completed and consecrated on February 22, 1898, in honor of the Holy Trinity.

After the revolution, the church was not destroyed. A significant contribution to its preservation was made by Anisya Afanasyevna Kopaneva. During the Great Patriotic War, the church was closed, but after the war, Anisya Afanasyevna achieved its reopening by gathering the signatures of nine communists, which was a true feat.

By the end of 1964, services in the temple ceased due to low income. The Krestovozdvizhenskaya church in Ankushino was closed in 1965 by the order of Bishop Ioann of Kirov.

After the church was closed, an enclosure for cows was set up inside, which erased the frescoes on the walls up to a height of one and a half meters. The village gradually became deserted, and by the end of the 1960s, the last residents had left.