The Church of the Assumption of the Blessed Virgin Mary was built in 1850 thanks to the funding of the local landowner, Princess Pelageya Nikolaevna Shirinskaya-Shikhmatova. This stone church with three chapels and a bell tower is designed in the Russian-Byzantine style by Konstantin Andreyevich Ton. The church is located in the center of the village, with its altar facing the main street. The churchyard, enclosed and rounded on the west side, includes a chapel and two gates located on the sides. All buildings are made of brick, originally covered with lime plaster, later they were plastered and painted in dark ochre with white decor.

The main chapel of the church is dedicated to the Assumption of the Mother of God, the right one to the prophet Elijah, and the left one to Saint Nicholas of Myra. The 1911 'Reference Book' mentions that the interior of the church was adorned with a copy of the miraculous icon of the Assumption of the Mother of God from the Kiev-Pechersk Lavra, gifted by Metropolitan Filaret of Kiev.

The church has preserved a two-tiered iconostasis with seven axes, made in the classical style but with eclectic elements such as applied carving. In the first tier, the columns have composite capitals, while in the second, twisted columns support the cornices. Most of the icon frames are arched. The iconostasis is painted blue, the columns are silvered, and the details are covered in bronze.

In the southern chapel, consecrated in honor of the prophet Elijah, there is a one-tiered iconostasis that corresponds in time to the main one. The iconostasis of the northern chapel of Saint Nicholas the Wonderworker has been partially preserved. Both chapel iconostases are painted blue with white details.

During the Soviet era, the church in Pechenkino was closed. It reopened after World War II and functioned until 1964, after which it was closed until 1990, when the church was returned to the faithful.