The church in the name of the Holy Trinity is located in the center of Troitskaya Street in the ancient Kremlin of Vladimir, in its northern part. The exact date of the church's founding is unknown, but it already existed in the early 17th century. The descriptive book of the Vladimir Kremlin from 1625 mentions "the temple of the Holy Life-Giving Trinity and the warm temple of St. Sergius, wooden, with church construction, royal doors, deesis, icons, books, candles, bells, and other church property."

On October 1, 1719, a fire occurred in Vladimir, resulting in the burning of the Trinity Church and the warm Sergius Church. The following year, the warm church was restored, while the construction of the cold church was likely postponed indefinitely due to a lack of funds.

By 1740, a stone church was built, and a warm chapel appeared in 1746. There is no information about the time of construction of the stone bell tower. The church has two altars: in the cold part in the name of the Life-Giving Trinity, and in the chapel in the name of St. Sergius. Among the icons, the Tolga Icon of the Mother of God and the icons of St. Sergius and St. Alexius, painted in ancient times, stand out.

Church documents have been preserved intact, including copies of metric books from 1817 and confession lists from 1849. There is no land or church houses for the clergy. According to the staff, there is a priest and a psalmist. The clergy receives support from the interest on a capital of 6182 rubles, as well as income from services and needs, totaling about 1000 rubles a year. The parish records list 208 men and 300 women, all Orthodox, residing in the city.