The warm brick church, located in the southern part of the monastery, was built between 1865 and 1869. This single-domed building was adjacent to a two-story cell building and had chapels dedicated to Mitrophan of Voronezh and Theodosius of Chernihiv. The church was closed no later than the 1920s, its dome was destroyed, and the building is now used as a garage.

To the south of the Tikhvin Cathedral, one can see a modest octagon and the dome of the warm church dedicated to the Holy Trinity. On the southern side of the church was a chapel in honor of St. Mitrophan, the Wonderworker of Voronezh, and on the northern side, in honor of St. Theodosius, the Wonderworker of Chernihiv. This church was a place of monastic prayer, where an atmosphere of godly and penitent prayer filled with humble joy reigned. Every corner served for spiritual solitude, and only the Queen of Heaven and Her Divine Son, the Lord Savior, knew the prayers that were raised here. The Trinity Church was directly connected to the cells of the nuns on the lower floor and to the cells of the abbess on the upper one. Below was a workshop for icon painting, where nine girls were trained under the guidance of a nun-master. Abbess Valentina sent the master to St. Petersburg for training in painting and gold icon stamping. The proximity of the church positively influenced the pious feelings of the icon painters and sometimes provided a convenient place for creating copies of icons. On the upper floor, near the cells of the abbess, there was a workshop for gold embroidery.