The Church of St. Nicholas in Mustvee is an Orthodox church located in Estonia and belongs to the Narva and Pskov Diocese of the Estonian Orthodox Church of the Moscow Patriarchate.

In 1839, a church named "Joy of All Who Sorrow" was built on the shore of the lake in the settlement of Chornom (the former name of Mustvee). It was also known as the Chornoselsky Church of All Who Sorrow. It was constructed from pine logs. A cemetery for Orthodox believers was organized next to the church. However, it soon became clear that the church was too small for all those wishing to attend services. As early as the 1840s, a petition was submitted to the diocesan authorities for the construction of a new stone church. In 1861, the construction of the church in honor of St. Nicholas the Wonderworker began, and it was completed in 1864. The consecration of the church took place in 1866.

The construction was financed by the treasury based on the project of diocesan architect A. Edelson, and the work was overseen by the contractor Vasily Grinkin from Chornov. The guarantor of the work was the Riga merchant Semyonov. The cost of construction amounted to 3,500 silver rubles. A two-tiered gilded iconostasis was installed in the church. The parish had a rectory and three schools: for boys and girls in Mustvee, as well as a supplementary school in Ray. The Chornov male parish school was opened in 1857, the Chornov female parish school in 1888, and the Ray supplementary school in 1860.

Currently, the rector of the parish is Hieromonk Ilya (Sorokaty).