The church was built in the late 17th and early 18th centuries, with its main altar dedicated to the Savior Not Made by Hands. Originally, a wooden church in honor of the Savior Not Made by Hands was erected in the mid-16th century after Ivan the Terrible's visit to the monastery. Among the gifts from the tsar for healing at the Tolgskaya icon was an icon of the Savior Not Made by Hands, which became a shrine of the monastery and was revered as miraculous.

In the 1710s, a small one-story stone church in honor of the Savior Not Made by Hands was built, with facades adorned with stucco and sculptural elements. The church's dome is topped with nine domes: one large and eight small. Initially, on the southern side of the church, there was an altar in honor of the Resurrection of the Righteous Lazarus, which was abolished in 1793 due to dilapidation, but in 1798 Count A. I. Osterman restored it and consecrated it in honor of St. Dimitry of Rostov.

A two-story building, known as the hospital wing, was added to the church from the west, where retired officers in need of care lived since 1757. In 1798, a monastery hospital was established here. In 1806, classes for the Yaroslavl Theological Seminary began to be held in the Savior Church, and later for the district parish school. In 1836, the dilapidated hospital wing was returned to the monastery, and during repairs, the second floor was dismantled. In 1880, the Savior Church was renovated, adding a new porch. Since 1888, auxiliary services, a refectory, a kitchen, a dishwashing area, and a bakery were located here. In 1911, the church was repaired: the walls were painted with oil paint, stone floors were replaced with wooden ones, and the domes were covered with aluminum. There were two entrances to the church, a ceremonial and an everyday one, from the side of the hospital wards.

On the wall of the former hospital wing, memorial plaques are placed in memory of those who found their final resting place under the church's refectory. Burial in the monastery was a privilege for people whose lives were marked by spiritual feats and service to the Fatherland. The names N. A. Tuchkov and A. P. Melgunov deserve special attention.

During the Soviet era, the Savior Church housed a kindergarten. In 1959, specialists from 'Yarrestavratsiya' noted that the church was in extremely poor condition, with cracks throughout the main volume of the building. In the 1960s, restoration work was carried out by 'Yarrestavratsiya', preventing its destruction. In 1985, a project was developed to adapt the Savior building into a recreation pavilion with a fireplace hall, a living room, and a cinema, but it was not implemented.

The restoration of the church began after the monastery was transferred to the Russian Orthodox Church. In 1988, the Savior Church was consecrated by Archbishop Platon of Yaroslavl and Rostov. The first iconostasis stood until 1988, with icons painted by Orthodox artist I. V. Vatagina from Moscow. In 1998, a new iconostasis was made according to the project of nun Tikhona Pospelova. The gilding of the iconostasis, carved kiots, and wall paintings were done by the sisters of the monastery. Restoration work was completed by 1999.