Eight kilometers north of Sergiev Posad, on the Uglich road, lies the village of Mishutino, home to the Church of the Nativity of the Blessed Virgin Mary. Mishutino is first mentioned in the spiritual charter of Grand Duke Ivan III as the center of the Mishutin estate. In documents from 1621-1622, it is referred to as the "ancient estate" of Yakov Gavrilovich Pisemsky, where a wooden church of the Nativity of the Blessed Virgin Mary was built with funds from landowners and peasants.

In 1699, part of the village passed into the ownership of Alexander Danilov, a sergeant of the Preobrazhensky Regiment and the son of Menshikov. In the early 18th century, the Trinity-Sergius Monastery owned the village, and from 1764, Mishutino became part of private lands. The Church of the Nativity of the Blessed Virgin Mary existed until 1781.

By the end of the 18th century, Mishutino had a wooden manor house and a wooden Spasskaya church with an altar in honor of the Nativity of the Blessed Virgin Mary. Since 1799, services in the church were not held due to its dilapidated condition. In 1805, a stone church with three altars was built with donations from parishioners: in the name of the All-Merciful Savior, in honor of the Nativity of the Blessed Virgin Mary, and the icon of the Mother of God "Joy of All Who Sorrow." The church housed a locally venerated icon of the Mother of God "The Passionate." In 1842, at the initiative of nobleman Alexei Gavrilovich Popov, a bell tower was built. The village had a church school and a chapel. In the 19th century, Mishutino was divided into two parts, owned by different landowners. In the 1880s, the church parish included the village and six villages with 855 parishioners. In 1923, there were 63 households in Mishutino.

From 1917 to 1928, Priest Pavel Preobrazhensky, a future holy martyr, served at the Church of the Nativity of the Blessed Virgin Mary. In 1928, he was appointed rector of the church in the village of Sinykovo in the Dmitrov district. On September 30, 1937, he was arrested on false charges of counter-revolutionary agitation and executed on October 21. In 1930, Mishutino became part of the collective farm named after the 2nd Five-Year Plan. In 1937, the church was closed, and the last rector, Hieromonk Lavrenty Nasonov, was arrested and shot on December 10, 1937, at the Butovo firing range near Moscow. The church building was converted into a grain dryer, and later a fire occurred there. During World War II, in 1943, a canteen was organized in the church, but another fire destroyed the interior of the church. In the 1970s, the church's dome collapsed.

Until 1991, the church in Mishutino remained abandoned, after which it was transferred to the Orthodox community, and restoration began. The first rector was Priest Alexei Savitsky. In 1993, Priest Alexander Zakharov became the rector, under whom the church began to transform. The bell tower, roof, floor, dome, and crosses were restored, heating was installed, and the churchyard was improved. In 2003, Archpriest Alexander Samoylov gifted the church an icon of the Resurrection of Christ. In 2005, the church celebrated its 200th anniversary.

In 2009, Priest Sergey Moshkov was appointed rector of the church.